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merck millipore,默克密理博,324865,PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb
产品名称：PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb
产品型号：324865
This PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).
merck millipore,默克密理博,324865,PhosphoDetect™ Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. 产品目录编号 324865 品牌系列 Calbiochem®
同义词 Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵) 应用数据 
Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.参考 参考 Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.产品信息 形式 Liquid 配方 In Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4. 正控制 A431 cells stimulated with EGF 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR 宿主 Rabbit 同种型 IgG 品种 human 储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-70°C).
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