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merck millipore,默克密理博,S7811,CpG WIZ hMLH1 - Methylation specific PCR assay
产品名称：CpG WIZ hMLH1 - Methylation specific PCR assay
产品型号：S7811
The components of the CpG WIZ hMLH1 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
merck millipore,默克密理博,S7811,CpG WIZ hMLH1 - Methylation specific PCR assay
- 产品介绍
描述 产品目录编号 S7811 品牌系列 Chemicon®
商名 - Chemicon
- CpG Wiz
描述 CpG WIZ® hMLH1 - Methylation specific PCR assay 概述 Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). The human mutL homolog 1 (hMLH1) gene exhibits characteristic hypermethylation.
Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.产品信息 部件 - Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C
- The components of the CpG WIZ® hMLH1 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
- U Primer Set7.5 μM each primer (25X) 35 μL (neutral cap) 90563 -15°C to -25°C
- M Primer Set7.5 μM each primer (25X) 35 μL (red cap) 90564 -15°C to -25°C
- W Primer Set7.5 μM each primer (25X) 35 μL (green cap) 90565 -15°C to -25°C
- U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C
- M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C
- W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C
应用 应用 The components of the CpG WIZ hMLH1 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples. 主要应用 - PCR
应用说明 Principles of the Technique
Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® HMLH1 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.生物信息 品种反应性 Human Entrez基因编号 - NM_000249.2
Entrez基因汇总 This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. 基因符号 - HNPCC2
- MLH1
- HNPCC
- COCA2
- FCC2
- hMLH1
- MGC5172
UniProt编号 - P40692
UniProt汇总 FUNCTION: SwissProt: P40692 # Involved in the repair of mismatches in DNA.
SIZE: 756 amino acids; 84601 Da
SUBUNIT: Heterodimer of MLH1 and PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
DISEASE: SwissProt: P40692 # Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2) [MIM:609310]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term suspected HNPCC or incomplete HNPCC can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. & Defects in MLH1 are a cause of Turcot syndrome [MIM:276300]. Turcot syndrome is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots. & Defects in MLH1 are a cause of Muir-Torre syndrome (MTS) [MIM:158320]. MTS is a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy. & Defects in MLH1 may contribute to lobular carcinoma in situ (LCIS), a non-invasive neoplastic disease of the breast. & Defects in MLH1 are a cause of susceptibility to endometrial cancer [MIM:608089].
SIMILARITY: SwissProt: P40692 ## Belongs to the DNA mismatch repair mutL/hexB family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 25 assays
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