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描述 概述 Recombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15 mM HCl, add 7.5 mM sterile NaOH and bring the pH to approximately 5.2. 产品目录编号 429700 品牌系列 Calbiochem®
同义词 rhOB 参考 参考 Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
Ookuma, M., et al. 1998. Diabetes 47, 219.
Campfield, L.A., et al. 1995. Science 269, 546.
Halaas, J.L., et al. 1995. Science 269, 543.
Pelleymounter, M.A., et al. 1995. Science 269, 540.
Zhang, Y., et al. 1994. Nature 372, 425.产品信息 CAS号 177404-21-6 形式 Lyophilized 配方 Lyophilized from a sterile filtered solution in PBS. Molar Mass 16000 生物信息 生物活性 ED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells 纯度 ≥97% by SDS-PAGE 理化信息 污染物 Endotoxin: ≤1.0 EU/µg leptin 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
特殊指南 To reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
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