merck millipore,默克密理博,04-229,Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal

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merck millipore,默克密理博,04-229,Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal
产品名称：Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal
产品型号：04-229
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-229,Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-229,Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, FC, ICC, IHC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-229
品牌系列	Upstate
商名	Upstate
描述	Anti-HDAC2 Antibody, clone Y461, rabbit monoclonal
Alternate Names	transcriptional regulator homolog RPD3 histone deacetylase 2 YY1-associated factor 1
背景信息	Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

产品信息
格式	Culture Supernatant
控制	K562 whole cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
应用说明	Flow Cytometry: 1:60 dilution of a previous lot was used on flow cytometry. Immunoprecipitation: 1:60 dilution of a previous lot was used on immunoprecipitation. Immunocytochemistry: 1:250-500 dilution of a previous lot was used in immunocytochemistry. Immunohistochemistry: 1:250-500 dilution of a previous lot was used in immunohistochemistry.

生物信息
免疫原品种	KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 2.
表位	C-terminus
克隆	Y461
宿主	Rabbit
特异性	This antibody recognizes Histone Deacetylase 2 at the C-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse and rat HDAC2.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001527
Entrez基因汇总	This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A,H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq].
基因符号	YAF1 EC 3.5.1.98 HD2 OTTHUMP00000040427 RPD3
纯化方法	Unpurified
UniProt编号	Q92769
UniProt汇总	FUNCTION: SwissProt: Q92769 # Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. SIZE: 488 amino acids; 55364 Da SUBUNIT: Interacts with the non-histone region of H2AFY (By similarity). Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, DNMT1, MINT, HDAC7, HDAC10, HCFC1, NRIP1, MJD2A/JHDM3A, SAP30, SETDB1 and SUV39H1. Interacts with the non-histone region of H2AFY. Interacts with PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with CBFA2T3. Interacts with SAP30L. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed; lower levels in brain and lung. SIMILARITY: SwissProt: Q92769 ## Belongs to the histone deacetylase family. Type 1 subfamily.

产品使用声明
质量保证	Routinely evaluated by Western Blot on K562 whole cell lysate. Western Blot Analysis: A 1:2000-1:10,000 dilution of this lot was used to detect Histone Deacetylase 2 on K562 whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.