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merck millipore,默克密理博,MAB2300X,Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated
产品名称：Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated
产品型号：MAB2300X
Milli-Mark FluoroPan Neuronal Marker -Alexa488 conjugated is an antibody targeting the FluoroPan Neuronal Marker protein, validated for use in ICC & IF.
merck millipore,默克密理博,MAB2300X,Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R ICC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 MAB2300X 商名 - Neuro-Chrom
描述 Milli-Mark™ FluoroPan Neuronal Marker - Alexa488 conjugated 背景信息 Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques, such as Golgi staining, and modern molecular approaches, such as GFP constructs, yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore previously developed a monoclonal antibody blend (Catalog No. MAB2300) that reacts against key somatic, nuclear, dendritic, spine, and axonal proteins distributed across the pan-neuronal architecture. Now Millipore has formulated MAB2300X, an Alexa 488 conjugated version of the pan-neuronal antibody blend MAB2300 to facilitate immunofluorescence studies. Each antibody within the blend is conjugated to Alexa 488 therefore enabling detection of an entire neuron with only one microscopic emission channel. MAB2300X has been validated in diverse fixations, cell culture, and immunohistochemistry protocols, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology. High morphological resolution has been achieved using MAB2300x including distral dentrites and spines. 产品信息 格式 AlexaFluor®488 控制 - Cortical neurons
演示 Blended monoclonal antibody cocktail in PBS with 0.05% NaN3 应用 应用 Milli-Mark FluoroPan Neuronal Marker -Alexa488 conjugated is an antibody targeting the FluoroPan Neuronal Marker protein, validated for use in ICC & IF. 主要应用 - Immunocytochemistry
应用说明 Immunocytochemistry:
1. Culture neurons as desired. When ready, gently wash with PBS and fix cells with 4% paraformaldehyde in PBS, 30min at room temp (RT).
2. Wash with PBS, 3 x 5min each, at RT and block with blocking buffer (1% BSA, 5% Serum, 0.2% Triton X in PBS) for 1 hr at RT.
3. Incubate cells with Milli-Mark FluoroPan Neuronal Marker (Mouse IgG conjugated with Alexa 488) for 2hr at RT (dilute antibody in blocking buffer).
4. Wash with PBS, 3 x 5min each, and cover slip with LIGHT DIAGNOSTICS Mounting Fluid (Catalog No. #5013). View using fluorescent microscope with FITC filter.
生物信息 表位 Whole Neuron Marker 宿主 Mouse 特异性 MAB2300X is specific to axons (neurites), dendrites, spines, nucleus, and the cell body of neurons. 同种型 IgG1 品种反应性 MouseHumanRat Species Reactivity Note Rat, Mouse, and Human. Reactivity with other species has not been determined. 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Routinely tested on cortical neurons in Immunocytochemistry 1:150 - 1:100.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 6 months from date of receipt. 包装信息 数量 100 µL
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