merck millipore,默克密理博,04-1158,Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal

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merck millipore,默克密理博,04-1158,Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal
产品名称：Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal
产品型号：04-1158
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1158,Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1158,Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1158
描述	Anti-Calcium/Calmodulin Kinase I Antibody, clone EPR2217Y, rabbit monoclonal
Alternate Names	calcium/calmodulin-dependent protein kinase kinase 2, beta CAMKK beta protein CaM-KK beta CaM-kinase kinase beta CaMKK beta Calcium/calmodulin-dependent protein kinase kinase beta calcium/calmodulin-dependent protein kinase beta calcium/calmodulin-dependent protein kinase kinase 2 beta
背景信息	The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

产品信息
格式	Unpurified
控制	Human brain, mouse brain, rat brain, and SHSY5Y lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Calcium/Calmodulin Kinase I in human kidney tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

生物信息
免疫原品种	Specific peptide corresponding to residues on the N-terminus of human Calcium/Calmodulin Kinase I.
表位	N-terminus
克隆	EPR2217Y
宿主	Rabbit
特异性	This antibody recognizes Calcium/Calmodulin Kinase I.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003647.1
Entrez基因汇总	Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activate calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
基因符号	MGC15254 CAMKK CAMKKB EC 2.7.11.17 KIAA0787
纯化方法	Unpurified
UniProt编号	Q96RR4
UniProt汇总	FUNCTION: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1 By similarity. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by Ca2+/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca2+/calmodulin. In part, activity is independent on Ca2+/calmodulin By similarity. SUBUNIT STRUCTURE: Interacts with calmodulin By similarity. SUBCELLULAR LOCATION: Cytoplasm By similarity. TISSUE SPECFICITY: Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. DOMAIN: The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition. The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates By similarity. PTM: Autophosphorylated By similarity. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. SEQUENCE CAUTION: The sequence CAD38990.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

产品使用声明
质量保证	Evaluated by Western Blot on Human brain, mouse brain, rat brain, and SHSY5Y lysates. Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect Calcium/Calmodulin Kinase I in Human brain, mouse brain, rat brain, and SHSY5Y lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.