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描述 概述 Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag® sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells. M.W. 65,000 and 30,000 under reducing conditions. 产品目录编号 499260 品牌系列 Calbiochem®
同义词 rmOPN 参考 参考 Xie, Y., et al. 2001. Kidney Int. 60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.产品信息 形式 Lyophilized 配方 Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN. 生物信息 生物活性 ~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well 纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotoxins: ≤100 pg/µg rmOPN 溶解度 Sterile PBS 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.
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