merck millipore,默克密理博,04-1027,Anti-SGK Antibody, clone Y238, rabbit monoclonal

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merck millipore,默克密理博,04-1027,Anti-SGK Antibody, clone Y238, rabbit monoclonal
产品名称：Anti-SGK Antibody, clone Y238, rabbit monoclonal
产品型号：04-1027
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-SGK Antibody, clone Y238, Rabbit detects level More>>

merck millipore,默克密理博,04-1027,Anti-SGK Antibody, clone Y238, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1027,Anti-SGK Antibody, clone Y238, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H, R	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1027
描述	Anti-SGK Antibody, clone Y238, rabbit monoclonal
Alternate Names	serum/glucocorticoid regulated kinase 1 Serum/glucocorticoid-regulated kinase 1 serine/threonine protein kinase SGK serum/glucocorticoid regulated kinase
背景信息	Serum and glucocorticoid-regulated kinase (SGK-1) is a 50 kDa Serine/Threonine kinase that plays a role in the regulation of epithelial Na+ transport. Two additional SGK isoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylation at Thr256 and Ser422 by the phosphatidyl-dependent protein kinase -1 (PDK1) and PDK-2 respectively. Nedd4-2 regulates SGK activity by catalyzing its ubiquination and degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+ channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+ transport. While environmental stresses increases its expression, SGK is known to play a role in promoting cell survival, proliferation and differentiation.

产品信息
格式	Unpurified
控制	A431 and NIH/3T3 cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-SGK Antibody, clone Y238, Rabbit detects level of SGK & has been published & validated for use in WB, IH(P), IP, FC.
主要应用	Flow Cytometry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected SGK in human kidney carcinoma tissue. Flow Cytometry: A 1:30 dilution from a previous lot was used in FC. Immunoprecipitation: A 1:50 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues of human SGK.
克隆	Y238
宿主	Rabbit
特异性	This antibody recognizes SGK.
同种型	IgG
品种反应性	MouseHumanRat
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_005618
Entrez基因汇总	This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such ascell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
基因符号	SGK EC 2.7.11.1 OTTHUMP00000017247
纯化方法	Unpurified
UniProt编号	O00141
UniProt汇总	FUNCTION: Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na+ transport than isoform 1. CATALTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Two specific sites, one in the kinase domain (Thr-256) and the other in the C-terminal regulatory region (Ser-422), need to be phosphorylated for its full activation. SUBUNIT STRUCTURE: Interacts with NEDD4 and NEDD4L. SUBCELLULAR LOCATION: Isoform 2: Cell membrane. Cytoplasm. Nucleus. Endoplasmic reticulum. Note: Nuclear, upon phosphorylation. TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle. INDUCTION: Isoform 1 is induced by serum and/or glucocorticoids. Isoform 2 is not induced by glucocorticoids. By excessive extracellular glucose and by TGF-beta, in cultured cells. DOMAIN: Isoform 2 subcellular localization at the plasma membane is mediated by the sequences within the first 120-amino acid. PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on A431 and NIH/3T3 cell lysates. Western Blot Analysis: A 1:500 dilution of this antibody was used to detect SGK in A431 and NIH/3T3 cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.