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品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, Chp IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6008 Replaces 04-1150 描述 Anti-MMP-9 Antibody, Hinge region Alternate Names - matrix metalloproteinase 9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- Gelatinase B
- macrophage gelatinase
- matrix metallopeptidase 9
背景信息 All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).
MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.产品信息 格式 Affinity Purified 控制 - PMA-treated HL-60 concentrated media.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect MMP-9 using this Anti-MMP-9 Antibody, Hinge region validated for use in WB, IH & IP. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:100 dilution detected MMP-9 in breast cancer ductal carcinoma tissue.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MMP-9 in HL-60 RIPA cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human MMP-9 at and around the hinge region. 表位 Hinge region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes MMP-9 at the hinge region. 品种反应性 MonkeyHumanChimpanzee Species Reactivity Note Demonstrated to react with human. Predicted to react with chimpanzee and monkey based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004985.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq] 基因符号 - MMP-9
- MMP9
- CLG4B
- GELB
纯化方法 Affinity Purfied UniProt编号 - P14780
UniProt汇总 FUNCTION: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
SIZE: 707 amino acids; 78427 Da
SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.产品使用声明 质量保证 Evaluated by Western Blot using a PMA-treated HL-60 concentrated media.
Western Blot Analysis: 5 µg/ml of this antibody detected MMP-9 in 10 µg of a PMA-treated HL-60 concentrated media.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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