merck millipore,默克密理博,04-1003,Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

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merck millipore,默克密理博,04-1003,Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal
产品名称：Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal
产品型号：04-1003
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1003,Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1003,Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1003
描述	Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal
Alternate Names	H3 histone, family 3A H3K9Ac Histone H3 (acetyl K9)
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

产品信息
格式	Unpurified
控制	Untreated and TSA-treated NIH/3T3 cell lysate.
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Histone H3 in human lung carcinoma tissue. Immunocytochemistry Analysis: A 1:250 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Acetylated-synthetic peptide corresponding to residues surrounding Lys9 of Histone H3.
表位	Acetylated Lys9
克隆	Y28
宿主	Rabbit
特异性	The antibody only detects Histone H3 acetylated on Lys9.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003493
基因符号	OTTHUMP00000035621 H3.3A H3.3B H3F3 MGC87782 MGC87783 OTTHUMP00000035618 OTTHUMP00000035619
修改	Acetylation
纯化方法	Unpurified
UniProt编号	P84243
UniProt汇总	FUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters. Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes. Ubiquitinated By similarity. SEQUENCE SIMILARITIES: Belongs to the histone H3 family. SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.

产品使用声明
质量保证	Evaluated by Western Blot in untreated and TSA-treated NIH/3T3 cell lysates. Western Blot Analysis: A 1:500 dilution of this antibody was used to detect Histone H3 in untreated and TSA-treated NIH/3T3 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.