merck millipore,默克密理博,03-102,RIPAb+ HuR - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-102,RIPAb+ HuR - RIP Validated Antibody and Primer Set
产品名称：RIPAb+ HuR - RIP Validated Antibody and Primer Set
产品型号：03-102
This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-102,RIPAb+ HuR - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-102,RIPAb+ HuR - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
R, H, M	IHC, WB, ICC

描述
产品目录编号	03-102
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ HuR - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction. The RIPAb+ HuR set includes the HuR antibody, a negative control antibody (purified Rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human beta actin (ACTB) cDNA. The HuR and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation HuR-associated RNAs.
Alternate Names	Hu-antigen R ELAV-like protein 1
背景信息	Hu-antigen R (HuB), ELAV-like protein 1, (ELAV1) is a transcription factor belonging to the ELAV family of RNP proteins. It binds avidly to the Au-rich element in cFos and interleukin-3 mRNAs.

产品信息
格式	Purified
控制	Included negative control rabbit IgG antibody and control primers specific for human beta actin (ATCB).
演示	Anti-HuR (rabbit polyclonal). One vial containing 50 μg of purified rabbit polyclonal in 100 μL of buffer containing PBS in 0.05% sodium azide with 50% Glycerol. Liquid at -20°C. Normal Rabbit IgG. One vial containing 125 μg purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, ACTB. One vial containing 75 μL of 5 μM of each primer specific for human beta actin (ACTB). Store at -20°C. FOR: TTG TTA CAG GAA GTC CCT TGC C REV: ATG CTA TCA CCT CCC CTG TGT G

应用
应用	This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Immunohistochemistry Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation from RIP lysate: RIP lysate from MCF7 cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-HuR antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HuR (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures). Western Blot Analysis: 3T3/A31 lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with HuR, hu (1:500 dilution). Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP and visualized using a chemiluminescence detection system. (Please see figures). Immunoprecipitation Analysis: 10 ug of this antibody immunoprecipitated HuR from 500 ug of 3T3/A31 RIPA lysate (Please see figures). mmunocytochemistry Analysis: Confocal IF analysis of HeLa, NIH/3T3 using anti-HuR (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue) (Please see figures).

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.
表位	a.a. 1-13 of HuR
宿主	Rabbit
特异性	HuR
品种反应性	RatHumanMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001410
Entrez基因汇总	The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.
基因符号	Hua ELAVL1 MelG HUR ELAV1 HuR
纯化方法	Protein A purfied
UniProt编号	Q15717
UniProt汇总	FUNCTION: SwissProt: Q15717 # Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. SIZE: 326 amino acids; 36092 Da SUBUNIT: Interacts with ANP32A. TISSUE SPECIFICITY: Ubiquitous. PTM: Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. SIMILARITY: SwissProt: Q15717 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	RNA binding protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 x 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgG or Anti-HuR antibody and the Magna RIP Kit (Cat. # 17-700). Successful immunoprecipitation of HuR-associated RNA was verified by qPCR using RIP Primers, ACTB (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA binding protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 x 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgG or Anti-HuR antibody and the Magna RIP Kit (Cat. # 17-700).
Successful immunoprecipitation of HuR-associated RNA was verified by qPCR using RIP Primers, ACTB (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.