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merck millipore,默克密理博,MABN695,Anti-CRTC2 Antibody, clone 5B10
产品名称：Anti-CRTC2 Antibody, clone 5B10
产品型号：MABN695
Anti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry More>>
merck millipore,默克密理博,MABN695,Anti-CRTC2 Antibody, clone 5B10
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC, WB, ICC, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABN695 描述 Anti-CRTC2 Antibody, clone 5B10 Alternate Names - Transducer of CREB protein 2
- TORC2
- TORC-2
- CRTC2
- CREB-regulated transcription coactivator 2
- Transducer of regulated cAMP response element-binding protein 2
背景信息 CREB-regulated transcription coactivator 2 (CRTC2) is a transcriptional coactivator for CREB1 which is a cAMP (CRE) site responder. CRTC2 is also known as TORC-2 (transducer of regulated cAMP response element-binding protein 2) and CRTC2 regulates gluconeogenesis as well as steroidogenic processes, and is also a coactivator for mitochondrial biogenesis in muscle cells. Localization is cytoplasmic but relocates to the nucleus in response to cAMP and glucagon levels. CRTC2 is highly expressed in thymus, spleen, muscle, and lung; it is also expressed in various cancers as well as insulinomas. Low levels of expression are found in the brain, colon, heart and kidney, with the lowest levels being in the liver and pancreas. Activity is regulated via phosphorylation. CRTC2 is inactive when phosphorylated (meaning that is does not bind CREB and assist in gene transcription) and active when dephosphorylated. EMD-Millipore’s Anti-CRTC2 mouse monoclonal, clone 5B10, has been tested in western blots against human HEK292 and HeLa cell extracts, in immunohistochemistry in paraffin embedded human ovary and lung cancer tissue, and in immunofluorescent immunocytochemistry and flow cytometry on HeLa cells in culture. 产品信息 格式 Ascites 控制 - HeLa cell lysate
演示 Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Anti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in HeLa cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in human lung cancer and ovarian tumor tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC2 in HeLa cells.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified protein fragment of human CRTC2 expressed in E. coli. 克隆 5B10 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_859066
基因符号 - CRTC2
- TORC2
- TORC-2
纯化方法 Unpurified UniProt编号 - Q53ET0
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CRTC2 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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