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merck millipore,默克密理博,06-702,Anti-phospho-Smad1 (Ser463/465) Antibody
产品名称：Anti-phospho-Smad1 (Ser463/465) Antibody
产品型号：06-702
Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
merck millipore,默克密理博,06-702,Anti-phospho-Smad1 (Ser463/465) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H, M, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-702 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-Smad1 (Ser463/465) Antibody 背景信息 Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways. 产品信息 格式 Purified 控制 - BMP-4-treated HeLa or NIH/3T3 cells
演示 Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative. 应用 应用 Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the dual serine phosphorylated Smad1 (Ser463/465). 同种型 IgG 品种反应性 XenopusHumanMouseRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005900
Entrez基因汇总 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. 基因符号 - BSP1
- SMAD1
- MADR1
- JV41
- JV4-1
- GARS
- CMT2D
- hSMAD1
- HMN5
- MADH1
- BSP-1
修改 - Phosphorylation
纯化方法 Protein A purfied UniProt编号 - Q15797
UniProt汇总 FUNCTION: SwissProt: Q15797 # Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
SIZE: 465 amino acids; 52260 Da
SUBUNIT: Interacts with HGS and NANOG (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
TISSUE SPECIFICITY: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
PTM: Phosphorylated on serine by BMP type 1 receptor kinase. & Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
SIMILARITY: SwissProt: Q15797 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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