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检测方法 Colorimetric 描述 概述 A non-isotopic, sandwich ELISA for the in vitro measurement of human MMP-9 in tissue culture supernatant, serum, or plasma. 产品目录编号 QIA56 品牌系列 Calbiochem®
同义词 Gelatinase B ELISA Kit, 92 kDa Gelatinase ELISA Kit, Matrix Metalloproteinase 9 ELISA Kit 应用数据 
The mean signal of each standard run in replicates of four in eight assays using two different lots of plates and two different lots of detector antibody.
The pooled coefficients of variation (according to the formula of Henry et. al., 1974) and between assay coefficients of variation are plotted against MMP-9 concentrations. The pooled data were collected from samples run eight times using two different lots of plates and two different lots of detector antibody in replicates of four on two separate occasions.
The study tested dilution-recovery of ten positive samples. The dilutions were run in the MMP-9 ELISA Kit and the "found" doses were plotted against the "expected" doses to determine linearity of dilution. The slope is not significantly different from one and the intercept is not significantly different from zero. These studies are consistent with the absence of cross-reacting and matrix effects such as pH, salts, and endogenous binders that interfere with the reagents used in the assay. Note: Ca = Cancer; NHS = Normal Human Sera; NHP = Normal Human Plasma; PMA = Phorbol 12-myristate 13-acetate; EGF = Epidermal Growth Factor; AR = Amphiregulin; Both = EGF and AR; A = 2 x 106 cells/ml; B = 1.0 x 106 cells/ml.
Levels of MMP-9 detected by the ELISA after immunoaffinity extraction of MMP-9 positive samples (PMA treated HT1080 and HL-60 cells) with a MMP-9 antibody that is not used in the ELISA, a TIMP-1 antibody, a TIMP-2 antibody and a TIMP-3 antibody.
APMA promotes the autocatalytic cleavage of the N-terminal propeptide of the latent 92-kDa enzyme to yield the active form of the enzyme. Analysis of the samples by zymography showed both cleavage of the proenzyme by APMA and a good correlation with the levels of the proenzyme determined with the MMP-9 ELISA. Several methods of MMP-9 induction were used to generate positive samples such as treatment with 50 ng/ml amphiregulin (AR), 50 ng/ml epidermal growth factor (EGF), 1 ng/ml transforming growth factor β (TGFβ) and 25 ng/ml phorbol 12-myristate 13-acetate (PMA). Normal human sera (NHS) containing moderate levels of MMP-9 were treated with APMA and analyzed.参考 参考 Massova, I., et al. 1998. FASEB J. 12, 1075.
Xie, B.,et al. 1998. J. Biol. Chem. 273, 11576.
Borden, P., and Heller, R. A. 1997. Crit. Rev. Eukaryot. Gen. Expression 7, 159.
Chambers, A. F., and Matrisian, L. M. 1997. J. Natl. Cancer Inst. 89, 1260.
DeClerck, Y.A., et al. 1997. Adv. Exp. Med. Biol. 425, 89.
Gomis-Ruth, F. X., et al. 1997. Nature 389, 77.
Murphy, G., and Knauper, V. 1997. Matrix Biol. 15, 511.
Olson, M. W., et al. 1997. J. Biol. Chem. 272, 29975.
Woodhouse, F. C., et al. 1997. Cancer 80, 1529.
Yu, A. E., et al. 1997. Drugs Aging 11, 229.
Coussens, L. M., and Werb, Z. 1996. Chem. Biol. 3, 895.
Lim Y.T., et al. 1996. J. Cell. Physiol. 167, 333.
Kohn, E. C., and Liotta, L. A. 1995. Cancer Res. 55, 1856.
Shapiro, S.D., et al. 1995. J. Biol. Chem. 270, 6351.
Murphy, G., et al. 1994. J. Biol. Chem. 269, 6632.
Fridman, R., et al. 1993. Biochem J. 289, 411.
Goldberg, G. L., et al. 1992. J. Biol. Chem. 267, 4583.
Morodomi T.,et al. 1992. Biochem. J. 285, 603.产品信息 检测方法 Colorimetric 声明 Not available for sale in Japan. 形式 34 or 41 Tests 格式 96-well plate 试剂盒包含 96-Well Coated Plate, MMP-9 Standard, Biotinylated Detector Antibody, 400X Streptavidin Peroxidase Conjugate, Diluent, Substrate, Assay Buffers, Stop Solution, Plate Sealer, and a user protocol. 生物信息 化验范围 0.625 - 20 ng/ml 化验时间 3.5 h 样品类型 Tissue culture media, serum, plasma 品种 human 理化信息 灵敏度 0.1 ng/ml 安全信息 R相 R: 23/24/25-35-36-40-43
Toxic by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes.
Limited evidence of a carcinogenic effect.
May cause sensitization by skin contact.S相 S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® MMP-9 ELISA Kit is a non-isotopic immunoassay for the in vitro quantitation of human MMP-9 protein in tissue culture medium, serum, and plasma. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival, store the entire contents at -20°C. Avoid multiple freeze/thaw cycles. Do not expose reagents to excessive light. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 96-Well Coated Plate, MMP-9 Standard, Biotinylated Detector Antibody, 400X Streptavidin Peroxidase Conjugate, Diluent, Substrate, Assay Buffers, Stop Solution, Plate Sealer, and a user protocol.
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