merck millipore,默克密理博,MAB3446,Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

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merck millipore,默克密理博,MAB3446,Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
产品名称：Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
产品型号：MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has More>>

merck millipore,默克密理博,MAB3446,Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

产品介绍

merck millipore,默克密理博,MAB3446,Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, ELISA	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MAB3446
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
Alternate Names	Chromobox Protein Homolog 5 HP1a Antigen p25
背景信息	Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

产品信息
格式	Ascites
控制	Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
演示	Liquid ascites fluid containing no preservative.

应用
应用	Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
主要应用	Western Blotting ELISA
应用说明	ELISA: 1:500-1:5,000 Western blot: 1:500-1:5,000 Immunoprecipitation: 1:500-1:5,000 Immunohistochemistry/Immunocytochemistry: Not Recommended Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Recombinant mouse HP1alpha.
克隆	2HP-2G9
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
同种型	IgG1
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_012117.1
Entrez基因汇总	Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM]
基因符号	HP1 CBX5 HP1Hs-alpha HP1-ALPHA HP1A
纯化方法	Unpurified
UniProt编号	P45973
UniProt汇总	FUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. SIZE: 191 amino acids; 22225 Da SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127. SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis. SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.