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品种反应性 主要应用 宿主 格式 抗体类型 Pm, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE52 Replaces 04-221 描述 Anti-Cyclin D Antibody Alternate Names - cyclin D1 (PRAD1: parathyroid adenomatosis 1)
- B-cell CLL/lymphoma 1
- BCL-1 oncogene
- G1/S-specific cyclin D1
- PRAD1 oncogene
- cyclin D1
背景信息 Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas. 产品信息 格式 Affinity Purified 控制 - 3T3/A31 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-Cyclin D Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP to detect Cyclin D also known as B-cell CLL/lymphoma 1, BCL-1 oncogene. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 5 µg/ml from a previous lot detected Cyclin D on 10 µg of 3T3/A31 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cyclin D in NIH/3T3, A431, C2C12, and HeLa cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Cyclin D at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Cyclin D at the C-terminus. 品种反应性 PrimateHumanMouse Species Reactivity Note Demonstrated to react with human and mouse. Predicted to react with primate based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_444284.1
Entrez基因汇总 The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]. 基因符号 - BCL1
- CCND1
- D11S287E
- U21B31
- PRAD1
纯化方法 Affinity Purfied UniProt编号 - P24385
UniProt汇总 FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition.
SIZE: 295 amino acids; 33729 Da
SUBUNIT: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
DISEASE: A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. & A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. & A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.
SIMILARITY: Belongs to the cyclin family. Cyclin D subfamily.产品使用声明 质量保证 Evaluated by Western Blot in 3T3/A31 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cyclin D on 10 µg of 3T3/A31 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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