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merck millipore,默克密理博,16-663,Magna ChIP™ Protein A+G Magnetic Beads
产品名称:Magna ChIP™ Protein A+G Magnetic Beads
产品型号:16-663
Magnetic A+G bead blend allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitation More>>
merck millipore,默克密理博,16-663,Magna ChIP™ Protein A+G Magnetic Beads
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重要规格表
Volume (mL) 1 描述 产品目录编号 16-663 描述 Magna ChIP™ Protein A+G Magnetic Beads 概述 Recombinant protein A plus recombinant protein G covalently bound to magnetic beads. This magnetic A+G blend allows for the use of a wider range of antibodies than A or G alone and provides a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitation (ChIP) assays as well as RNA immunoprecipitation (RIP) assays. Compared with conventional agarose beads, magnetic beads significantly reduce handling times and mechanical stress on target immunocomplexes. Alternate Names - ChIP magnetic beads, ChIP magnetic A/G beads, ChIP magnetic A+G beads
- ChIP magnetic A/G beads
- ChIP magnetic beads
背景信息 Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays. 产品信息 演示 Liquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide. 应用 应用 Magnetic A+G bead blend allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays. 应用说明 Use 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex. 尺寸 粒径(µm) ~3 体积(mL) 1 产品使用声明 质量控制测试 Routinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP™ A Kit (Cat. #17-610). 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of shipment. Do Not Freeze. 储存温度(°C) 2–8 (Do not freeze) 包装信息 数量 50 reactions
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