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merck millipore,默克密理博,MABT130,Anti-Protein disulfide-isomerase Antibody, clone 1D3
产品名称：Anti-Protein disulfide-isomerase Antibody, clone 1D3
产品型号：MABT130
This Anti-Protein disulfide-isomerase Antibody, clone 1D3 is validated for use in WB, IC for the detection of Protein disulfide-isomerase.
merck millipore,默克密理博,MABT130,Anti-Protein disulfide-isomerase Antibody, clone 1D3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABT130 描述 Anti-Protein disulfide-isomerase Antibody, clone 1D3 Alternate Names - p55
- Protein disulfide-isomerase
- PDI
- Cellular thyroid hormone-binding protein
- Prolyl 4-hydroxylase subunit beta
背景信息 Protein disulfide-isomerase, also known as PDI, is an abundant component of the ER that catalyzes disulfide bond formation in the biosynthesis of various secretory and cell surface proteins. It is a multifunctional polypeptide identical to the β-subunit of prolyl 4-hydroxylase, a α2β2 tetramer that catalyzes the formation of 4-hydroxyproline in collagen by the hydroxylation of proline residues in peptide linkages. PDI has two thioredoxin-like regions, each containing a Cys-Gly-His-Cys sequence that may represent two independently acting catalytic sites of PDI activity. The C-terminus of PDI has a KDEL sequence, which acts as retention signal for proteins of the ER lumen. The PDI/β-subunit of prolyl-4-hydroxylase is required for its retention in the ER. 产品信息 格式 Purified 控制 - Human adrenal gland tissue lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-Protein disulfide-isomerase Antibody, clone 1D3 is validated for use in WB, IC for the detection of Protein disulfide-isomerase. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in methanol-fixed NRK cells (Vaux, D., et al. (1990). Nature. 345(7):495-502). 生物信息 免疫原品种 Linear peptide corresponding to human Protein disulfide-isomerase. 克隆 1D3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000909
Entrez基因汇总 This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]. 基因符号 - PO4DB
- P4HB
- ERBA2L
- PDI
- PDIA1
纯化方法 Protein G Purified UniProt编号 - P07237
UniProt汇总 FUNCTION: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
CATALYTIC ACTIVITY: Catalyzes the rearrangement of -S-S- bonds in proteins.
SUBUNIT STRUCTURE: Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity (By similarity). Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex.
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein Potential. Note: Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources Probable. Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
MISCELLANEOUS: Reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell.
SEQUENCE SIMILARITIES: Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.产品使用声明 质量保证 Evaluated by Western Blot in human adrenal gland tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Protein disulfide-isomerase in 10 µg of human adrenal gland tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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