merck millipore,默克密理博,MABS155,Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	M	Unconjugated	Monoclonal Antibody

描述
产品目录编号	MABS155
描述	Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
Alternate Names	G-protein coupled receptor kinase 2 Beta-adrenergic receptor kinase 1 Beta-ARK-1
背景信息	G-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context.

产品信息
格式	Unconjugated
控制	Untreated and Calyculin A/Okadaic Acid treated A431 cells
演示	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.
主要应用	Western Blotting
应用说明	Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells. Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670.
表位	Phosphorylated Ser670
克隆	15G11.1
宿主	Mouse
特异性	This antibody recognizes GRK2 phosphorylated at Ser670.
同种型	IgG2bκ
品种反应性	Human
Species Reactivity Note	Demonstrated to react with Human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001610
基因符号	GRK2 ADRBK1 BARK BARK1
纯化方法	Protein G purified
UniProt编号	P25098

产品使用声明
质量保证	Evaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells. Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MABS155,Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech