merck millipore,默克密理愽
  • merck millipore,默克密理博,71346,Origami™ 2(DE3)pLysS Competent Cells - Novagen

  • merck millipore,默克密理博,71346,Origami™ 2(DE3)pLysS Competent Cells - Novagen

    产品名称：Origami™ 2(DE3)pLysS Competent Cells - Novagen
    产品型号：71346
    Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.

    merck millipore,默克密理博,71346,Origami™ 2(DE3)pLysS Competent Cells - Novagen

  • 产品介绍
  • merck millipore,默克密理博,71346,Origami™ 2(DE3)pLysS Competent Cells - Novagen

    描述
    概述 Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.

    DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

    pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
    Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB pLysS (CamR, StrR, TetR)
    产品目录编号 71346
    品牌系列 Novagen®
    产品信息
    部件
    保证高效 2 × 10⁶ cfu/µg
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Multiple Toxicity Values, refer to MSDS
    储存 ≤ -70°C
    无需冷冻 No
    布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

    merck millipore,默克密理博,71346,Origami™ 2(DE3)pLysS Competent Cells - Novagen

上一件merck millipore产品：merck millipore,默克密理博,230699,Chondroitin Sulfate, Bovine Trachea - CAS 9007-28-7 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,CBL1337F,Anti-PECAM-1 Antibody, clone 390, FITC conjugated


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