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品种反应性 主要应用 宿主 格式 抗体类型 H, R, M IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN461 描述 Anti-Dicer1 Antibody, clone N167/7 Alternate Names - Double-strand-specific ribonuclease mDCR-1
- Endoribonuclease Dicer
背景信息 RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC). 产品信息 格式 Purified 控制 - MCF-7 cell lysate
演示 Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Dicer1 antibody is validated for use in WB & IHC for the detection of the Dicer1 protein. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dicer1 in human ovarian adenocarcinoma tissues and in rat cerebellum tissue.
Western Blot Analysis: A representative lot from an independent laboratory detected Dicer1 in adult rat brain membrane and in WT mouse brain lysate, and demonstrated a loss of signal in Dicer1 knockout mouse brain lysate.生物信息 免疫原品种 Recombinant protein corresponding to mouse Dicer1. 克隆 N167/7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 HumanRatMouse Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_683750
基因符号 - Mdcr
- Dicer
纯化方法 Protein G purified UniProt编号 - Q8R418
产品使用声明 质量保证 Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Dicer1 in 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MABN461,Anti-Dicer1 Antibody, clone N167/7
下一件merck millipore产品：merck millipore,默克密理博,MABE290,Anti-Estrogen Receptor β Antibody, clone 9.88
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