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merck millipore,默克密理博,MABE290,Anti-Estrogen Receptor β Antibody, clone 9.88
产品名称：Anti-Estrogen Receptor β Antibody, clone 9.88
产品型号：MABE290
Use Anti-Estrogen Receptor β Antibody, clone 9.88 (Mouse Monoclonal Antibody) validated in WB, IP to detect Estrogen Receptor β also known as Estrogen receptor beta.
merck millipore,默克密理博,MABE290,Anti-Estrogen Receptor β Antibody, clone 9.88
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, R, M WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE290 描述 Anti-Estrogen Receptor β Antibody, clone 9.88 Alternate Names - Nuclear receptor subfamily 3 group A member 2
- Estrogen receptor beta
- ER-beta
背景信息 Estrogen receptor beta (ERβ; NR3A2 or ESR2) is a nuclear steroid receptor that has been detected in the ovary, testis, thymus, spleen, bone, brain, and kidney. It is a multidomain protein containing A/B, C, D, E, and F domains, and it is activated by estrogen, resulting in homo- or heterodimerization (with ERα). The active ERβ-estrogen complex binds to regulatory regions known as estrogen response elements on target genes; however, ERβ may also regulate transcription without a bound ligand via its activation function (AF-1) region in the A/B domain. The ERβ receptor interacts with a wide variety of proteins including transcriptional corepressors and coactivators that regulate its activity. Its expression is also regulated by hypermethylation of CpG islands in the ERβ promoter. Previous studies have reported that ERβ has anti-inflammatory and anti-proliferative effects, and may therefore be an important therapeutic target for cancers, especially those occurring in the reproductive organs. 产品信息 格式 Purified 控制 - PC-12 cell lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Use Anti-Estrogen Receptor β Antibody, clone 9.88 (Mouse Monoclonal Antibody) validated in WB, IP to detect Estrogen Receptor β also known as Estrogen receptor beta. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Estrogen Receptor β in HUVEC cells (Simoncini, T., et al. (2006). Mol Endocrinol. 20(8):1756-1771.). 生物信息 免疫原品种 Recombinant protein corresponding to human Estrogen Receptor β. 克隆 9.88 宿主 Mouse 同种型 IgMκ 品种反应性 HumanRatMouse Species Reactivity Note Demonstrated to react with Human, Rat, and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001035365
Entrez基因汇总 This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. 基因符号 - NR3A2
- ESR2
- ESTRB
纯化方法 Purified by ion-exchange chromatography. UniProt编号 - Q92731
UniProt汇总 FUNCTION: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
SUBUNIT STRUCTURE: Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1 and UBE1C. Isoform beta-2/cx preferentially forms a hetereodimer with ESR1 rather than ESR2 and inhibits DNA-binding by ESR1. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PRMT2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
SEQUENCE CAUTION: The sequence CAA67555.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.产品使用声明 质量保证 Evaluated by Western Blot in PC-12 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor β in PC-12 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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