merck millipore,默克密理愽
  • merck millipore,默克密理博,MABF120,Anti-EGFR Antibody, clone 225 (Azide-free)

  • merck millipore,默克密理博,MABF120,Anti-EGFR Antibody, clone 225 (Azide-free)

    产品名称：Anti-EGFR Antibody, clone 225 (Azide-free)
    产品型号：MABF120
    Anti-EGFR Antibody, clone 225 (Azide-free) is an antibody against EGFR for use in Immunoprecipitation, Neutralizing.

    merck millipore,默克密理博,MABF120,Anti-EGFR Antibody, clone 225 (Azide-free)

  • 产品介绍
  • merck millipore,默克密理博,MABF120,Anti-EGFR Antibody, clone 225 (Azide-free)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HNEUT, IPMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABF120
    Replaces 04-338
    描述 Anti-EGFR Antibody, clone 225 (Azide-free)
    Alternate Names
    • Receptor tyrosine-protein kinase erbB-1
    • Epidermal growth factor receptor
    • Proto-oncogene c-ErbB-1
    背景信息 The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.
    产品信息
    格式 Purified
    控制
    • A431 cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
    应用
    应用 Anti-EGFR Antibody, clone 225 (Azide-free) is an antibody against EGFR for use in Immunoprecipitation, Neutralizing.
    主要应用
    • Neutralizing
    • Immunoprecipitation
    应用说明 Neutralizing Assay Analysis: A representative lot was used by an independent laboratory in A431 cells. (Kawamoto, T., et al. (1984). Journal of Biological Chemistry. 259(12):7761-7766.)
    生物信息
    免疫原品种 Partially purified human EGFR from A431 cells
    克隆 225
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    同种型 IgG1κ
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_005219
    Entrez基因汇总 The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010].
    基因符号
    • HER1
    • EGFR
    • ERBB
    • ERBB1
    纯化方法 Protein G purified
    UniProt编号
    • P00533
    UniProt汇总 FUNCTION: Isoform 2/truncated isoform may act as an antagonist.

    SIZE: 1210 amino acids; 134277 Da

    SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.

    SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.

    TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.

    PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE: Defects in EGFR are associated with lung cancer [MIM:211980].

    SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.

    MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
    产品使用声明
    质量保证 Evaluated by Immunoprecipitation in A431 cell lysate.

    Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated EGFR from A431 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MABF120,Anti-EGFR Antibody, clone 225 (Azide-free)

上一件merck millipore产品：merck millipore,默克密理博,MAB1535,Anti-Hexokinase Type III Antibody, clone C7C3
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