merck millipore,默克密理博,17-650,ChIPAb+ Ubiquityl-Histone H2B

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merck millipore,默克密理博,17-650,ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
产品型号：17-650
This ChIPAb+ Ubiquityl-Histone H2B -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-650,ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-650,ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Pl, M, H, Vrt, R	ChIP, WB

描述
产品目录编号	17-650
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Ubiquityl-Histone H2B, (clone 56) set includes the Ubiquityl-Histone H2B, (clone 56) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the human GAPDH gene. The ubiquityl-Histone H2B and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Ubiquityl-Histone H2B associated chromatin.
Alternate Names	histone cluster 1, H2bc histone 1, H2bc H2B.1 A H2B histone family, member L Histone H2B (ubiquityl) H2BUb

产品信息
格式	Protein G Purified
控制	Includes negative control mouse IgG antibody and primers specific for human GAPDH coding region.
演示	Anti-ubiquityl-Histone H2B, clone 56 (mouse monoclonal IgG). One vial containing 25 μg of protein G purified antibody in 25 μL PBS containing 0.05% sodium azide. Store at -20°C. Normal Mouse IgG. One vial containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C. ChIP Primers GAPDH Coding Region. One vial containing 75 μL of 5 μM of each primer specific for the coding region of human GAPDH. Store at -20°C. FOR: GGC TCC CAC CTT TCT CAT CC REV: GGC CAT CCA CAG TCT TCT GG

应用
应用	This ChIPAb+ Ubiquityl-Histone H2B -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 μg of either a normal mouse IgG or Anti-Ubiquityl-Histone H2B antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of ubiquityl-Histone H2B associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and GAPDH coding (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Ubiquityl-histone H2B at a dilution of 1:4000 (Please see figures).

生物信息
免疫原品种	Immunogen used was a branched peptide corresponding to the ubiquitination site on human histone H2B.
表位	Lys120 ubiquitination site
宿主	Mouse
特异性	Recognizes histone H2B ubiquitinated on lysine 120 but not free ubiquitin or histone H2B in its unmodified state.
同种型	IgG2aκ
品种反应性	Green PlantsMouseHumanVertebratesRat
Species Reactivity Note	Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003528.2
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
基因符号	H2BFL H2B.1 H2B.a/g/h/k/l H2B/a H2B/g H2B/h H2B/k H2B/l H2BFA H2BFG H2BFH H2BFK
修改	Ubiquitylation
UniProt编号	P62807
UniProt汇总	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-120 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. SEQUENCE SIMILARITY: Belongs to the histone H2B family.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa S3 cells (1X 10⁶ cell equivalents per IP) were subjected to chromatin immuno-precipitation using 1 µg of either a normal mouse IgG or Anti-ubiquityl-Histone H2B, clone 56 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of ubiquityl-Histone H2B-associated DNA fragments was verified by qPCR using Control Primers. Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1X 10⁶ cell equivalents per IP) were subjected to chromatin immuno-precipitation using 1 µg of either a normal mouse IgG or Anti-ubiquityl-Histone H2B, clone 56 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of ubiquityl-Histone H2B-associated DNA fragments was verified by qPCR using Control Primers.
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.