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merck millipore,默克密理博,04-889,Anti-Src Antibody, clone N6L, rabbit monoclonal
产品名称：Anti-Src Antibody, clone N6L, rabbit monoclonal
产品型号：04-889
This Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src.
merck millipore,默克密理博,04-889,Anti-Src Antibody, clone N6L, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-889 Replaces 05-889 描述 Anti-Src Antibody, clone N6L, rabbit monoclonal Alternate Names - v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog<br />(avian)
- proto-oncogene tyrosine-protein kinase SRC
- protooncogene SRC, Rous sarcoma
- tyrosine kinase pp60c-src
- tyrosine-protein kinase SRC-1
- v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene<br />homolog
背景信息 pp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype 产品信息 格式 Culture Supernatant 控制 - Included positive antigen control of non-stimulated A431 lysate (Cat. No. 12-301). Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Cultured supernantant containing 0.05% sodium azide. 应用 应用 This Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src. 主要应用 - Western Blotting
生物信息 免疫原品种 N-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose. 克隆 N6L 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn. 同种型 IgG 品种反应性 Human Species Reactivity Note Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005408.1
Entrez基因汇总 This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.基因符号 - pp60c-src
- ASV
- EC 2.7.10.2
- OTTHUMP00000174476
- OTTHUMP00000174477
- SRC1
- c-SRC
- c-Src
- c-src
- p60-Src
UniProt编号 - P12931
UniProt汇总 CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 By similarity. Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. Interacts with CAV2 (tyrosine phosphorylated form).
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
产品使用声明 质量保证 Western Blot Analysis: A 1:500 to 1:1000 dilution of this antibody detected Src in RIPA lysates from A431 cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µl
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