merck millipore,默克密理博,FC010-100MG,Human Plasma Fibronectin Purified Protein

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merck millipore,默克密理博,FC010-100MG,Human Plasma Fibronectin Purified Protein
产品名称：Human Plasma Fibronectin Purified Protein
产品型号：FC010-100MG

merck millipore,默克密理博,FC010-100MG,Human Plasma Fibronectin Purified Protein

产品介绍

merck millipore,默克密理博,FC010-100MG,Human Plasma Fibronectin Purified Protein

重要规格表

主要应用
CULT

描述
产品目录编号	FC010-100MG
Replaces	FC010-250MG
品牌系列	Chemicon®
商名	Chemicon
描述	Human Plasma Fibronectin Purified Protein
概述	Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

产品信息
演示	Lyophilized from 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, containing no preservatives.

应用
主要应用	Cell Culture
应用说明	Immunochemical standard Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts Suggested Procedure for Coating Cell Cultureware 1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2. 2. Reconstitute the entire vial* of hFN to 1mg/mL using distilled or deionized water at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1). 3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area. 4. Introduce the proper CO2 atmosphere, if required. 5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below. 6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes. 7. Remove residual balanced salt solution before proceeding with standard cell culture procedures. *Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.

应用

主要应用

Cell Culture

应用说明

Immunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2.

2. Reconstitute the entire vial* of hFN to 1mg/mL using distilled or deionized water at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1).

3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

4. Introduce the proper CO2 atmosphere, if required.

5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below.

6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes.

7. Remove residual balanced salt solution before proceeding with standard cell culture procedures.

*Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.

生物信息
纯度	Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
来源	Human donor plasma.
品种	Human
Entrez基因编号	NM_212482.1 NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1
Entrez基因汇总	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
基因符号	FINC FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS
UniProt编号	P02751
UniProt汇总	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain lyophilized material at -20°C for up to 12 months from date of receipt. Store the reconstituted material at 2º-8° C for up to 6 months.