merck millipore,默克密理博,MABE317,Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A	ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABE317
描述	Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01
Alternate Names	5'OHmeC 5hmeC 5' hydroxymethyl cytosine 5'OH methyl cytosine
背景信息	5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

产品信息
格式	Purified
控制	Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Detect 5-Hydroxymethylcytosine (5hmC) using this Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01 validated for use in Dot Blot, ICC, Immunoprecipitation.
主要应用	Immunocytochemistry
应用说明	Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Analysis: A representative lot was used by an independent laboratory to immunoprecipitate 5-Hydroxymethylcytosine DNA usingh chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences) Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

生物信息
免疫原品种	BSA-conjugated recombinant Hydroxymethylcytosine DNA.
克隆	HMC-MA01
宿主	Mouse
特异性	This antibody detects 5-Hydroxymethylcytosine, but not 5-Methylcytosine or unmethylated cytosine.
同种型	IgG1κ
品种反应性	All
Species Reactivity Note	Expected to detect 5-Hydroxymethylcytosine in all species
抗体类型	Monoclonal Antibody
纯化方法	Protein G purified

产品使用声明
质量保证	Evaluated by Dot Blot in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-Hydroxymethylcytosine DNA. Dot Blot Analysis: A 1:5000 dilution of this antibody detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MABE317,Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone HMC-MA01

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