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品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABC128 描述 Anti-FIP200 Antibody, clone 14E11.2 Alternate Names - FIP200
- RB1-inducible coiled-coil protein
- 1FAK family kinase-interacting protein of 200 kDa
背景信息 Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the interaction domain of human FIP200. 表位 Interaction domain 克隆 14E11.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions. 同种型 IgG2bκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001077086
基因符号 - RBICC
- RB1CC1
- KIAA0203
纯化方法 Protein G purified UniProt编号 - Q86YR4
产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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