merck millipore,默克密理博,04-1109,Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal

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merck millipore,默克密理博,04-1109,Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal
产品名称：Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal
产品型号：04-1109
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1109,Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1109,Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1109
描述	Anti-Integrin beta1 [CD29] Antibody, clone EP1041Y, rabbit monoclonal
Alternate Names	integrin, beta 1 (fibronectin receptor, beta polypeptide,<br />antigen CD29 includes MDF2, MSK12) CD29 antigen Fibronectin receptor subunit beta Integrin VLA-4 subunit beta fibronectin receptor beta subunit integrin VLA-4 beta subunit integrin beta 1
背景信息	The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and a beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family is based upon the pairing abilities of the individual alpha and beta subunits. Adhesion results in integrin aggregation into structures called focal adhesion plaques, which form the link between the extracellular space and the intracellular cytoskeleton. Signaling from focal adhesions is initiated by the kinases FAK, Pyk2 and ILK. Phosphorylation and cleavage of integrins result in modifications of cell adhesion, permitting physiological processes such as migration, vascular proliferation, and wound repair. Integrin beta 1, also known as CD29, is a transmembrane glycoprotein that forms complexes with many integrin alpha subunits, (1 – 11), to form functional receptors that bind to specific extracellular matrix proteins.

产品信息
格式	Unpurified
控制	U937 cell lysate.
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Flow Cytometry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected Integrin beta1 in human breast carcinoma tissue. Flow Cytometry: 1:70 dilution from a previous lot was used in FC.

生物信息
免疫原品种	Synthetic peptide corresponding to the residue near the C-terminal of human Integrin beta1.
表位	C-terminus
克隆	EP1041Y
宿主	Rabbit
特异性	This antibody recognizes Integrin beta1
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P05556
基因符号	VLAB CD29 FNRB GPIIA MDF2 MSK12 OTTHUMP00000019420 OTTHUMP00000046253 OTTHUMP00000063731 OTTHUMP00000063732 OTTHUMP00000063733 VLA-BETA
纯化方法	Unpurified
UniProt编号	P05556
UniProt汇总	FUNCTION: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Interacts with FLNA, FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with KRT1 in the presence of GNB2L1 and SRC. Interacts with HIV-1 Tat. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses. Interacts with RAB21. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note: Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in afew intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle). PTM: The cysteine residues are involved in intrachain disulfide bonds By similarity. SEQUENCE SIMILARITIES: Belongs to the integrin beta chain family. Contains 1 VWFA domain.

产品使用声明
质量保证	Evaluated by Western Blot on U937 cell lysate. Western Blot Analysis: 1:500 dilution of this antibody was used to detect Integrin beta1 in U937 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.