merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB88078,Anti-Heat Shock Factor I Antibody, clone 10H8

  • merck millipore,默克密理博,MAB88078,Anti-Heat Shock Factor I Antibody, clone 10H8

    产品名称：Anti-Heat Shock Factor I Antibody, clone 10H8
    产品型号：MAB88078
    This Anti-Heat Shock Factor I Antibody, clone 10H8 is validated for use in ELISA, IF, IP, WB for the detection of Heat Shock Factor I.

    merck millipore,默克密理博,MAB88078,Anti-Heat Shock Factor I Antibody, clone 10H8

  • 产品介绍
  • merck millipore,默克密理博,MAB88078,Anti-Heat Shock Factor I Antibody, clone 10H8

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Mk, H, MWB, ELISARPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB88078
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Heat Shock Factor I Antibody, clone 10H8
    Alternate Names
    • HSFI
    产品信息
    格式 Purified
    控制
    • POSITIVE CONTROL: NIH-3T3, K562, HL-60, HEP-2, or HeLa cells
    演示 Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
    应用
    应用 This Anti-Heat Shock Factor I Antibody, clone 10H8 is validated for use in ELISA, IF, IP, WB for the detection of Heat Shock Factor I.
    主要应用
    • Western Blotting
    • ELISA
    应用说明 I mmunofluorescence (predominantly nuclear)

    Immunoprecipitation: 10 μL/mg cell lysate

    Western Blot: 1:200-1:400 (for 2hrs. at Room Temperature)

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Full length recombinanat mouse HSF1 protein (aa 1-503).
    克隆 10H8
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rat
    特异性 MAB88078 recognizes a protein of 70-85 kDa (depending upon the souce and state of cells), identified as Heat Shock Factor 1 (HSF1). Reactivity with delection mutants suggests that the epitope of MAB88078 spans in the aa 378-395 of mouse HSF1. The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediate by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosophila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. The phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.

    Cellular Localization: Cytoplasmic domain
    同种型 IgG1
    品种反应性 MonkeyHumanMouse
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_005526.2
    Entrez基因汇总 The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.
    基因符号
    • HSTF1
    • HSF1
    UniProt编号
    • Q00613
    UniProt汇总 FUNCTION: SwissProt: Q00613 # DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    SIZE: 529 amino acids; 57260 Da
    SUBUNIT: DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a monomer. Interacts with SYMPK and CSTF2 in heat-stressed cells.
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.
    SIMILARITY: SwissProt: Q00613 ## Belongs to the HSF family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,MAB88078,Anti-Heat Shock Factor I Antibody, clone 10H8

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