merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3623,Anti-Caspase 3 Antibody, active (cleaved) form

  • merck millipore,默克密理博,AB3623,Anti-Caspase 3 Antibody, active (cleaved) form

    产品名称：Anti-Caspase 3 Antibody, active (cleaved) form
    产品型号：AB3623
    Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 & has been published & validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), & Western Blot (WB).

    merck millipore,默克密理博,AB3623,Anti-Caspase 3 Antibody, active (cleaved) form

  • 产品介绍
  • merck millipore,默克密理博,AB3623,Anti-Caspase 3 Antibody, active (cleaved) form

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3623
    Replaces 04-440; 04-1088
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Caspase 3 Antibody, active (cleaved) form
    Alternate Names
    • procaspase3
    • Apopain
    • Yama Protein
    • CASP3
    • SCA-1
    背景信息 Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
    产品信息
    格式 Affinity Purified
    控制
    • Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.
    演示 Purified rabbit polyclonal in buffer containing PBS, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
    应用
    应用 Anti-Caspase 3 Antibody, active (cleaved) form detects level of Caspase 3 & has been published & validated for use in Immunofluorescence (IF), Immunohistochemistry (IHC), & Western Blot (WB).
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Western Blot Analysis:
    1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
    Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.

    Immunohistochemistry:
    1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.

    Immunohistochemistry(paraffin):
    Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
    生物信息
    免疫原品种 Cleavage specific synthetic peptide sequence (proprietary).
    表位 active (cleaved) form
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.
    品种反应性 RatHumanMouse
    Species Reactivity Note Human, Mouse, Rat. Reactivity with other species has not been determined.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_004346.3
    Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
    基因符号
    • EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
    • CASP3
    • CPP-32
    • Yama
    • CASP-3
    • CPP32B
    • SCA-1
    • CPP32
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P42574
    UniProt汇总 FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
    SIZE: 277 amino acids; 31608 Da
    SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
    SUBCELLULAR LOCATION: Cytoplasm.
    TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
    PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB3623,Anti-Caspase 3 Antibody, active (cleaved) form

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