merck millipore,默克密理博,04-974,Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal

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merck millipore,默克密理博,04-974,Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
产品名称：Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
产品型号：04-974
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, R More>>

merck millipore,默克密理博,04-974,Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-974,Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-974
描述	Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, rabbit monoclonal
Alternate Names	focal adhesion kinase 1 FADK 1 PTK2 protein tyrosine kinase 2 Protein-tyrosine kinase 2
背景信息	Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.

产品信息
格式	Unpurified
控制	Human and rat brain lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-phospho-FAK (Tyr397) Antibody, clone EP2160Y, Rabbit detects level of phospho-FAK (Tyr397) & has been published & validated for use in WB.
主要应用	Western Blotting

生物信息
免疫原品种	Phospho-specific peptide surrounding tyrosine 397 of human FAK.
表位	Tyr397
克隆	EP2160Y
宿主	Rabbit
特异性	This antibody detects FAK phosphorylated at Tyr397.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q05397
基因符号	pp125FAK EC 2.7.10.2 FADK FAK FAK1
修改	Phosphorylation
纯化方法	Unpurified
UniProt编号	Q05397
UniProt汇总	FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1. SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity. The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. PTM: Phosphorylated on 6 tyrosine residues upon activation. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on Human and rat brain cell lysates. Western Blot Analysis: 1:1,000-2,000 dilution of this antibody was used to detect FAK in human and rat brain cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.