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merck millipore,默克密理博,04-119,Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal
产品名称：Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal
产品型号：04-119
This Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).
merck millipore,默克密理博,04-119,Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Vrt, H ChIP, WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-119 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal Alternate Names - Histone H4 (acetyl K12)
- H4K12Ac
产品信息 格式 Culture Supernatant 演示 100 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide. 应用 应用 This Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit is validated for use in WB for the detection of acetyl-Histone H4 (Lys12). 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)生物信息 免疫原品种 Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated. 宿主 Rabbit 特异性 Recognizes Histone H4 when acetylated on Lys12. 同种型 IgG 品种反应性 VertebratesHuman Species Reactivity Note Reacts with Human, wide range of cross-reactivity expected based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_003538.3
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. 基因符号 - H4/E
- HIST1H4A
- H4/J
- HIST1H4F
- HIST1H4L
- H4FN
- H4FA
- H4FH
- HIST1H4H
- H4F2
- H4FM
- H4/A
- H4FK
- H4FG
- HIST2H4
- H4FB
- H4/K
- HIST1H4I
- H4/N
- HIST1H4B
- H4FE
- H4/M
- H4/a
- HIST1H4E
- HIST4H4
- HIST2H4A
- H4FC
- H4FI
- H4/H
- HIST1H4C
- H4/G
- HIST1H4K
- H4FJ
- H4FD
- H4/I
- H4/B
- H4/D
- H4/C
- HIST1H4J
- HIST1H4D
修改 - Acetylation
UniProt编号 - P62805
UniProt汇总 FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.产品使用声明 质量保证 Routinely evaluated by immunoblot. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C from date of shipment 包装信息 数量 100 µL
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