merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3560P,Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

  • merck millipore,默克密理博,AB3560P,Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

    产品名称：Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1
    产品型号：AB3560P
    Detect Sodium using this Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 validated for use in ELISA, IH & WB.

    merck millipore,默克密理博,AB3560P,Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

  • 产品介绍
  • merck millipore,默克密理博,AB3560P,Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, H, B, RWB, ELISA, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3560P
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1
    Alternate Names
    • solute carrier family 12 sodium/potassium/chloride transporters), member 2
    • Basolateral Na-K-Cl symporter
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
    • solute carrier family 12 sodium/potassium/chloride transporters)
    背景信息 The Na+/K+/Cl- Cotransporter isoform 1 (NKCC1) is involved in the transport of sodium, potassium, and chloride ions across membranes. NKCC1 mediates sodium and chloride reabsorption and plays an important role in cell volume regulation. NKCC1 is also involved in the control of normal cell proliferation, while its overexpression results in apparent cell transformation, in a manner similar to some protooncogenes (Panet, 1999). NKCC1 is inhibited by bumetanide and is also referred to as the bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1. Inhibition of NKCC1 activity by bumetanide decreases the high-[K+]o-mediated uptake of Cl , blocks astrocyte swelling, and leads to cell shrinkage under isotonic conditions in cells such as retinal pigment epithelia, ventricular myocytes, and vascular endothelial cells (Russell, 2000; Su, 2002). NKCC1 could contribute to an overload of intracellular Na+, K+, and Cl- and cell swelling in conditions such as cerebral ischemia (Yan, 2001). NKCC1 is expressed in multiple tissues with the amount of mRNA expression varying as much as thirty-fold. It is highly expressed in neurons and astrocytes through-out the brain and has been implicated in ischemic necrotic cell death.
    产品信息
    格式 Affinity Purified
    控制
    • Spinal cord, colon.
    演示 Affinity purified rabbit polyclonal in buffer containing PBS with 0.1% BSA and 0.05% sodium azide.
    应用
    应用 Detect Sodium using this Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1 validated for use in ELISA, IH & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunohistochemistry
    应用说明 Immunohistochemistry:
    It is recommended that the antibody be tried at 2-20 µg/mL on paraformaldehyde fixed tissue.

    ELISA:
    0.5-1.0 µg/mL of a previous lot was used with 1 µg/mL control peptide per well.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 A 22 amino acid peptide sequence near the C-terminus of rat NKCC1. (Moore-Hoon et al. 1998; Delpire et al. 1994; Payne et al. 1995; Isering et al. 1997; Mount et al. 1998).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes rat Sodium, Potassium, Chloride Cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.
    品种反应性 MouseHumanBovineRat
    Species Reactivity Note Rat. Expected to cross-react with human and mouse. The immunogen sequence is 95% conserved in mouse, 86% in human, and 82% in bovine.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_009194.3
    Entrez基因汇总 By moving chloride into epithelial cells, the Na-K-Cl cotransporter SLC12A2 aids transcellular movement of chloride across both secretory and absorptive epithelia (Payne et al., 1995 [PubMed 7629105]). See also SLC12A1 (MIM 600839) and SLC12A3 (MIM 600968).[supplied by OMIM]
    基因符号
    • BSC
    • SLC12A2
    • BSC2
    • NKCC1
    • MGC104233
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P55012
    UniProt汇总 FUNCTION: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
    SIZE: 1,205 amino acids; 130,950 Da
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in many tissues.
    PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
    SIMILARITY: Belongs to the SLC12A transporter family.
    产品使用声明
    质量保证 Evaluated by Western Blot on Rat Spinal Cord lysates.

    Western Blot Analysis:
    1:500 dilution detected NA+K+CL- COTRANS 1 on 10 µg of Rat Spinal Cord lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB3560P,Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

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