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merck millipore,默克密理博,AB1520,Anti-Glutamate Transporter Antibody, neuronal
产品名称:Anti-Glutamate Transporter Antibody, neuronal
产品型号:AB1520
Anti-Glutamate Transporter Antibody, neuronal detects level of Glutamate Transporter & has been published & validated for use in IH.
merck millipore,默克密理博,AB1520,Anti-Glutamate Transporter Antibody, neuronal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, Gp, M IHC Gt Serum Polyclonal Antibody 描述 产品目录编号 AB1520 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Glutamate Transporter Antibody, neuronal Alternate Names - EAAT3
- EAAC1
产品信息 格式 Serum 演示 Goat antiserum. Liquid, no preservatives. 应用 应用 Anti-Glutamate Transporter Antibody, neuronal detects level of Glutamate Transporter & has been published & validated for use in IH. 主要应用 - Immunohistochemistry
不建议的应用 - Western Blotting
应用说明 Immunohistochemistry: 1:4,000-8,000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material is recommended. The use of triton X-100 in the block only is recommended.
This antibody is not recommended for use in Western blot.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the carboxy-terminus of the cloned rat EAAC1. 宿主 Goat 特异性 Neuronal Glutamate Transporter (EAAC1; also called EAAT3). The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained with AB1520 corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preabsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining. 品种反应性 RatGuinea PigMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004170.4
基因符号 - EAAT3
- SLC1A1
- EAAC1
UniProt编号 - P43005
UniProt汇总 FUNCTION: SwissProt: P43005 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5 (By similarity).
SIZE: 524 amino acids; 57100 Da
SUBUNIT: Interacts with ARL6IP5/PRAF3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
PTM: Glycosylated.
DISEASE: SwissProt: P43005 # Defects in SLC1A1 may be a cause of dicarboxylicamino aciduria [MIM:222730]; also known as glutamate-aspartate transport defect. This is as defect in renal and probably intestinal transport of glutamic and aspartic acids and is associated with moderate hyperprolinemia.
SIMILARITY: SwissProt: P43005 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to one year.
Avoid repeated freeze/thaw cycles.包装信息 数量 50 µL
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