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品种反应性 主要应用 宿主 格式 抗体类型 Ch WB, ELISA M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB5240 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Phosphacan Antibody 背景信息 Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth. 产品信息 格式 Culture Supernatant 演示 Tissue culture supernatant. Liquid. Contains no preservative. 应用 应用 Detect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P). 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1:1,000
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunohistochemistry: 1:20 Works on cold methanol or paraformaldehyde fixed tissues. Works on paraffin embedded tissues. Chondrotinase treatment of tissue consists of treating mounted tissue section with a solution of PBS plus 0.5U/mL of Chondroitinase ABC for 30 minutes at room temperature prior to blocking and antibody staining.
Immunocytochemistry: not tested
ELISA: Excellent for core and monomer protein.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Mixture of chicken chondroitin sulfate proteoglycans. 宿主 Mouse 特异性 Phosphacan (Receptor Tyrosine Phosphatase Beta). 同种型 IgM 品种反应性 Chicken 抗体类型 Monoclonal Antibody 基因符号 - PTPZ
- PTPRZ1
- PTPRZ
UniProt编号 - Q62656 (rat)
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 1 mL
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