merck millipore,默克密理博,16-638,4G10 Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A	IP	M	Gel Immobilized	Monoclonal Antibody

描述
产品目录编号	16-638
商名	Upstate
描述	4G10® Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate
背景信息	The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10® Platinum. We pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone is capable.

产品信息
格式	Gel Immobilized
演示	Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10® (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.

应用
应用	4G10 Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
主要应用	Immunoprecipitation

生物信息
免疫原品种	The immunogen for 4G10® was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
表位	phosphotyrosine
克隆	4G10®
宿主	Mouse
特异性	Tyrosine-phosphorylated proteins from all species.
同种型	IgG2bκ
品种反应性	All
抗体类型	Monoclonal Antibody
纯化方法	protein G purified mouse monoclonal 4G10® (IgG2bκ) and protein A purified PY20 (IgG2b)

产品使用声明
质量保证	Immunoprecipitation: Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. NOTE: DO NOT FREEZE.

包装信息
数量	200 µL

merck millipore,默克密理博,16-638,4G10 Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate

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