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merck millipore,默克密理博,MAB5224,Anti-Internexin α Antibody, CT, clone 2E3
产品名称：Anti-Internexin α Antibody, CT, clone 2E3
产品型号：MAB5224
This Anti-Internexin Antibody, α, C-terminus, clone 2E3 is validated for use in ELISA, IP, WB, IH(P) for the detection of Internexin.
merck millipore,默克密理博,MAB5224,Anti-Internexin α Antibody, CT, clone 2E3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ma, H WB, ELISA M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB5224 Replaces 04-1032 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Internexin α Antibody, CT, clone 2E3 背景信息 The protein alpha-internexin is a ~66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits {Pachter J et al J. Cell Biol 101:1316-22 (1985). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration {McGraw et al 2002}. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. 产品信息 格式 Culture Supernatant 演示 Mouse IgG in low serum (1%), tissue culture supernatant containing no preservative. 应用 应用 This Anti-Internexin Antibody, α, C-terminus, clone 2E3 is validated for use in ELISA, IP, WB, IH(P) for the detection of Internexin. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: antibody detects a protein band at 55-66kDa, as expected for alpha internexin {Evans, et al, 2001}.
Immunocytochemistry on PC12 and Ntera-2 cells.
Immunohistochemistry on formalin and methanol fixed tissues. Works on paraffin embedded tissue sections.
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Full length recombinant rat alpha internexin. 表位 C-terminus 克隆 2E3 宿主 Mouse 特异性 Alpha internexin. Localized to the C-terminal 164 amino acids of rat alpha internexin. 同种型 IgG1 品种反应性 MammalsHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_032727.2
基因符号 - Alpha-Inx
- INA
- NF-66
- NEF5
- TXBP-1
- Neurofilament-66
- MGC12702
- Alpha-internexin
UniProt编号 - Q16352
UniProt汇总 FUNCTION: SwissProt: Q16352 # Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
SIZE: 499 amino acids; 55391 Da
TISSUE SPECIFICITY: Found predominantly in adult CNS.
DEVELOPMENTAL STAGE: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
PTM: O-glycosylated (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q16352 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 300 µL
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