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merck millipore,默克密理博,09-243,Anti-phospho JAK2 (Tyr972) Antibody
产品名称：Anti-phospho JAK2 (Tyr972) Antibody
产品型号：09-243
Anti-phospho JAK2 (Tyr972) Antibody detects level of phospho JAK2 (Tyr972) & has been published & validated for use in WB.
merck millipore,默克密理博,09-243,Anti-phospho JAK2 (Tyr972) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H, R, Eq, Op, B, Chp WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-243 描述 Anti-phospho JAK2 (Tyr972) Antibody Alternate Names - tyrosine-protein kinase JAK2
- Janus kinase 2
- Janus kinase 2 (a protein tyrosine kinase)
背景信息 JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. 产品信息 格式 Affinity Purified 控制 - Transfected 293T cell lysate treated with SH2B1 beta
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-phospho JAK2 (Tyr972) Antibody detects level of phospho JAK2 (Tyr972) & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 2 µg/mL from a previous lot detected phosphorylated JAK2 (Tyr972) in 10 µg of pervanadate-treated L6 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the protein kinase 2 domain of human JAK2. 表位 Protein kinase 2 domain, phosphorylated at Tyr972. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes phosphorylated JAK2 at Tyr972. 品种反应性 PigHumanRatHorseOpossumBovineChimpanzee Species Reactivity Note Demonstrated to react in human and rat.
Predicted to react with equine, opossum, bovine, chimpanzee, porcine, platypus, and ape based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004963
Entrez基因汇总 This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq]. 基因符号 - JTK10
- JAK-2
纯化方法 Affinity Purfied UniProt编号 - O60674
UniProt汇总 FUNCTION:Plays a role in leptin signaling and control of body weight by similarity. Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction.
CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with SIRPA and SH2B1 by similarity. Interacts with IL23R, SKB1 and STAM2.
SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein by similarity. Note: Wholly intracellular, possibly membrane associated by similarity.
TISSUE SPECIFICITY: Expressed in blood, bone marrow and lymph node.
DOMAIN: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain by similarity, while the presence of slight differences suggest a different role for domain 1.
PTM:Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 by similarity.
INVOLVEMENT IN DISEASE: Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain, and abdominal ascites.
Defects in JAK2 are associated with polycythemia vera (PV) [MIM:263300]. PV, the most common form of primary polycythemia, is caused by somatic mutation in a single hematopoietic stem cell leading to clonal hematopoiesis. PV is a myeloproliferative disorder characterized predominantly by erythroid hyperplasia, but also by myeloid leukocytosis, thrombocytosis, and splenomegaly. Familial cases of PV are very rare and usually manifest in elderly patients.
Defects in JAK2 gene may be a cause of essential thrombocythemia (ET) [MIM:187950]. ET is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
Defects in JAK2 are associated with familial myelofibrosis [MIM:254450]. Myelofibrosis with myeloid metaplasia is a myeloproliferative disease with annual incidence of 0.5-1.5 cases per 100,000 individuals and age at diagnosis around 60 (an increased prevalence is noted in Ashkenazi Jews). Clinical manifestations depend on the type of blood cell affected and may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension.
Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.产品使用声明 质量保证 Evaluated by Western Blot in transfected 293T cell lysate treated with SH2B1 beta.
Western Blot Analysis: 0.1 µg/mL of this antibody detected phosphorylated JAK2 (Tyr972) in 10 µg of 293T cells transfected with WT JAK2 and treated with SH2B1 beta, and less so in 293T cells transfected with mutant JAK2 (phenylalanine) and treated with SH2B1 beta.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB5224,Anti-Internexin α Antibody, CT, clone 2E3
下一件merck millipore产品：merck millipore,默克密理博,428018,Sodium n-Dodecyl Sulfate, 20% Solution (w/v) - CAS 151-21-3 - Calbiochem
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