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merck millipore,默克密理博,05-379,Anti-E2F-1 Antibody, clones KH20 and KH95
产品名称：Anti-E2F-1 Antibody, clones KH20 and KH95
产品型号：05-379
Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB.
merck millipore,默克密理博,05-379,Anti-E2F-1 Antibody, clones KH20 and KH95
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-379 品牌系列 Upstate
商名 - Upstate
描述 Anti-E2F-1 Antibody, clones KH20 and KH95 背景信息 E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. 产品信息 格式 Purified 控制 - Positive Control Included: HeLa Nuclear Extract (12-309)
演示 Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide. 应用 应用 Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST fusion protein corresponding to full-length human E2F-1. 表位 The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386. 克隆 KH20 & KH95 宿主 Mouse 特异性 Recognizes E2F-1. 同种型 IgG 品种反应性 XenopusHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005225
Entrez基因汇总 The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. 基因符号 - PBR3
- E2F1
- RBAP-1
- E2F-1
- RBP3
- RBBP3
- RBBP-3
纯化方法 Protein G purfied UniProt编号 - Q01094
UniProt汇总 FUNCTION: SwissProt: Q01094 # Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
SIZE: 437 amino acids; 46920 Da
SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T- antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
SIMILARITY: SwissProt: Q01094 ## Belongs to the E2F/DP family.产品使用声明 质量保证 Immunoblot Analysis: 0.5-2μg/ml of this antibody detected E2F-1 in a HeLa nuclear extract.
Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
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