• merck millipore,默克密理博,220477,Chitosanase, Streptomyces sp. N174
  • merck millipore,默克密理博,220477,Chitosanase, Streptomyces sp. N174

    产品名称:Chitosanase, Streptomyces sp. N174
    产品型号:220477


    merck millipore,默克密理博,220477,Chitosanase, Streptomyces sp. N174

  • 产品介绍
  • merck millipore,默克密理博,220477,Chitosanase, Streptomyces sp. N174

    重要规格表

    描述
    概述 Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
    产品目录编号 220477
    品牌系列 Calbiochem®
    同义词 Chitosan N-acetylglucosaminohydrolase
    参考
    参考 Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
    Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
    Fukamizo, T., et al. 1995. Biochem J. 311, 377.
    Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
    Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
    Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
    产品信息
    CAS号 51570-20-8
    EC号 3.2.1.132
    形式 Liquid
    配方 In 50 M sodium acetate, 50% glycerol, pH 5.0.
    Molar Mass 29500
    PI 7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
    生物信息
    特异活性 ≥25 units/mg protein
    理化信息
    污染物 chitinolytic activity: ≤0.001%, proteases: ≤0.01%
    pH值 optimum 5.5
    储存和货运信息
    货运代码 Blue Ice Only
    毒性 Standard Handling
    储存 -20°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    特殊指南 Following initial thaw, aliquot and freeze (-20°C).

    merck millipore,默克密理博,220477,Chitosanase, Streptomyces sp. N174

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