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描述 概述 Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. 产品目录编号 220477 品牌系列 Calbiochem®
同义词 Chitosan N-acetylglucosaminohydrolase 参考 参考 Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.产品信息 CAS号 51570-20-8 EC号 3.2.1.132 形式 Liquid 配方 In 50 M sodium acetate, 50% glycerol, pH 5.0. Molar Mass 29500 PI 7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C 生物信息 特异活性 ≥25 units/mg protein 理化信息 污染物 chitinolytic activity: ≤0.001%, proteases: ≤0.01% pH值 optimum 5.5 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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