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merck millipore,默克密理博,MAB4130,Anti-Thymidylate Synthase Antibody, clone TS106
产品名称：Anti-Thymidylate Synthase Antibody, clone TS106
产品型号：MAB4130
Use Anti-Thymidylate Synthase Antibody, clone TS106 (Mouse Monoclonal Antibody) validated in ELISA, FC, IF, IHC, IHC(P), IP, WB to detect Thymidylate Synthase also known as Thymidylate synthase.
merck millipore,默克密理博,MAB4130,Anti-Thymidylate Synthase Antibody, clone TS106
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA, FC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4130 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Thymidylate Synthase Antibody, clone TS106 Alternate Names - Thymidylate synthase
背景信息 TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival. 产品信息 格式 Purified 控制 - Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10
演示 Purified mouse monoclonal IgG1 in 0.02M PBS with 0.25M NaCl, pH=7.6, 0.1% sodium azide. 应用 应用 Use Anti-Thymidylate Synthase Antibody, clone TS106 (Mouse Monoclonal Antibody) validated in ELISA, FC, IF, IHC, IHC(P), IP, WB to detect Thymidylate Synthase also known as Thymidylate synthase. 主要应用 - Western Blotting
- ELISA
- Flow Cytometry
- Immunohistochemistry
应用说明 ELISA:
A previous lot of this antibody was used in ELISA.
Flow Cytometry:
A previous lot of this antibody was used in FC.
Immunofluorescence:
A previous lot of this antibody was used in IF.
Immunoprecipitation:
A 10 μL/mg concentration of a previous lot was used on protein lysate.
Immunohistochemistry (Frozen and Paraffin sections):
1:50 - 1:100 (with incubation for 30-60 minutes at room temperature). TS106 can benefit from antigen retrieval with either EDTA pH 8.0 or 10mM citrate buffer pH 6.0 .
Western Blotting:
1:200 - 1:400. Recognizes a 36KDa band.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant Thymidylate Synthase. 克隆 TS106 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Antibody MAB4130 recognizes a 36 kDa protein identified as Thymidylate Synthase [TS], which is a target for the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. Expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas. 同种型 IgG1 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse.
Expected to react with rat based on sequence homology.抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001071.1
Entrez基因汇总 Thymidylate synthase (TYMS, or TS; EC 2.1.1.45) uses the 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor to maintain the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.[supplied by OMIM] 基因符号 - EC 2.1.1.45
- TYMS
- TS
- Tsase
- TSase
- TMS
- HsT422
- MGC88736
纯化方法 Protein A purfied UniProt编号 - P04818
UniProt汇总 SIZE: 313 amino acids; 35716 Da
SUBUNIT: Homodimer.
SIMILARITY: SwissProt: P04818 ## Belongs to the thymidylate synthase family.产品使用声明 质量保证 Evaluated by Western Blot on MCF7 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected THYMIDYLATE SYNTHASE on 10 μg of MCF7 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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