merck millipore,默克密理博,MAB88916-C,Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free

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merck millipore,默克密理博,MAB88916-C,Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
产品名称：Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
产品型号：MAB88916-C
This Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free is validated for use in western blotting, IHC, ELISA, ICC & inhibition for the detection of Fibronectin.

merck millipore,默克密理博,MAB88916-C,Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free

产品介绍

merck millipore,默克密理博,MAB88916-C,Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB88916-C
描述	Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
Alternate Names	Cold-insoluble globulin CIG FN Fibronectin
背景信息	Fibronectin (FN), or in earlier literature also known as Cold-insoluble globulin (CIG), and is encoded by the gene named FN1/FN is an extracellular matrix protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Fibronectin also plays an important role in bone development and bone structure formation. Additionally, when fibronectin binds the protein anastellin it forms a multiprotein complex termed superfibronectin that has been shown to inhibit tumor growth, angiogenesis and metastasis. Fibronectin protein can be found extracellularly as well as intracellularly. There are multiple isoforms produced by alternative splicing. Plasma fibronectin (a soluble dimeric form) is secreted by hepatocytes into the blood. Cellular fibronectin (dimeric or cross-linked multimeric forms), are made by fibroblasts, epithelial and other cell types, and is deposited as fibrils in the extracellular matrix.

产品信息
格式	Purified
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

应用
应用	This Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free is validated for use in western blotting, IHC, ELISA, ICC & inhibition for the detection of Fibronectin.
主要应用	Immunocytochemistry Western Blotting Immunohistochemistry
应用说明	Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of U-251 cell lysate. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Fibronectin (Cell Attachment Fragment) in human placenta and liver tissues. ELISA Analysis: A representative lot detected the conformation of Fibronectin adsorbed onto self-assembled monolayers (SAMs) of alkanethiols over a range of Fibroectin surface densities (Kesolowsky, B. G., et al. (2003). J Biomed Mater Res A. 66(2):247-259.). Immuncytochemistry Analysis: A representative lot detected Fibronectin (Cell Attachment Fragment) in Extracellular matrix deposited by HUVECs (Orecchia, A., et al. (2003). J Cell Sci. 116(Pt 17):3479-3489.). Inhibition Analysis: A representative lot blocked cell-adhesive epitopes of Fibronectin (Cell Attachment Fragment) (Miller, D. C., et al. (2005). J Biomed Mater Res A. 73(4):476-84.). Inhibition Analysis: A representative lot blocked RGD-dependent cell adhesion (Tsang, T. M., et al. (2012). J Biol Chem. 287(20):16759-16767.). Inhibition Analysis: A representative lot inhibited the cell-adhesive epitopes of Fibronectin (Miller, D. C., et al. (2005). J Biomed Mater Res. 73(4):476-484.)

生物信息
免疫原品种	Purified human Fibronectin, which contains the Cell Attachment Fragment, from amnion liquid.
表位	This antibody recognizes the cell attachment fragment of Fibronectin.
克隆	3E3
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG1κ
品种反应性	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002017
基因符号	FN FN1
纯化方法	Protein G purified
UniProt编号	P02751

产品使用声明
质量保证	Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of HepG2 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.