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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB380 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tubulin β Antibody, clone 5H1 产品信息 格式 Ascites 演示 Ascites fluid containing sodium azide. Liquid 应用 应用 Anti-Tubulin Antibody, β, clone 5H1 is an antibody against Tubulin for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry at 1:300-1:1,000
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB380
This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).生物信息 免疫原品种 Rat tubulin. 克隆 5H1 宿主 Mouse 特异性 Beta tubulin 同种型 IgM 品种反应性 RatBovineHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006086.2
基因符号 - beta-4
- TUBB3
- TUBB4
- MC1R
UniProt编号 - Q13509
UniProt汇总 FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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