merck millipore,默克密理博,420092,PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb

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Merck Millipore 默克密理愽

宿主
Rb

描述
概述	Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
概述	Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
产品目录编号	420092
品牌系列	Calbiochem®
应用数据	Detection of mouse JAK1, phosphorylated on Tyr^1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr^1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

参考
参考	Liu, K.D., et al. 1997. Curr. Biol. 7, 817. Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.

产品信息
声明	Manufactured by Biosource International.
形式	Liquid
配方	In Dulbecco's PBS (without Ca²⁺ and Mg²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.
正控制	3T3L1 cells treated with LIF
防腐剂	≤0.1% sodium azide

应用
主要应用	Immunoblotting (Western Blotting)

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
免疫原品种	Human
宿主	Rabbit
同种型	IgG
品种	human, mouse, not rat

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	≤ -70°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following intitial thaw, aliquot and freeze (-70°C).

merck millipore,默克密理博,420092,PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb

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