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merck millipore,默克密理博,MAB3749,Anti-LEF-1 Antibody, transactivation domain, clone 3A12
产品名称：Anti-LEF-1 Antibody, transactivation domain, clone 3A12
产品型号：MAB3749
This Anti-LEF-1 Antibody, transactivation domain, clone 3A12 is validated for use in WB for the detection of LEF-1.
merck millipore,默克密理博,MAB3749,Anti-LEF-1 Antibody, transactivation domain, clone 3A12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3749 Replaces 04-1130 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-LEF-1 Antibody, transactivation domain, clone 3A12 Alternate Names - Lymphoid Enhancer Factor 1
背景信息 Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), andorthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants. 产品信息 格式 Purified 演示 Purified immunoglobulin. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide. 应用 应用 This Anti-LEF-1 Antibody, transactivation domain, clone 3A12 is validated for use in WB for the detection of LEF-1. 主要应用 - Western Blotting
应用说明 Western blot
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells. 表位 transactivation domain 克隆 3A12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_016269.2
Entrez基因汇总 Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM] 基因符号 - DKFZp586H0919
- LEF1
- LEF-1
- TCF1-alpha
- TCF1ALPHA
UniProt编号 - Q9UJU2
UniProt汇总 FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
SIZE: 399 amino acids; 44201 Da
SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,UFC30GV00,Ultrafree-MC，GV 0.22 µm
下一件merck millipore产品：merck millipore,默克密理博,IL032,Interleukin-12 Protein, Recombinant mouse
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