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品种反应性 主要应用 宿主 格式 抗体类型 Eq, H, R, M, Ch, Ca WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-437 描述 Anti-phospho-IRS1 (Ser1101) Antibody Alternate Names - insulin receptor substrate 1
背景信息 Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. 产品信息 格式 Affinity Purified 演示 Antigen affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl, with 0.05% NaN3. 应用 应用 Detect phospho-IRS1 (Ser1101) using this Anti-phospho-IRS1 (Ser1101) Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to amino acids 1097-1107 of IRS1. 表位 Phosphorylated IRS1 (Ser1101) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody will also detect phosphorylated IRS2 (Ser1149) based on sequence homology. 品种反应性 HorseHumanRatMouseChickenCanine Species Reactivity Note Human. Expected to react with Rat, Mouse, Horse, Chicken and Dog based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005535
基因符号 - HIRS-1
- IRS-1
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - P35568
UniProt汇总 FUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit By similarity.
SUBUNIT STURCTURE: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts By similarity. Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor By similarity.
POLYMORPHISM: of Tyr-896 is required for GRB2-binding By similarity.
Polymorphism The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.
INVOLVEMENT IN DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
产品使用声明 质量保证 Evaluated by Western Blot on untreated and insulin treated lysate from immunoprecipitated IRS1 transfected CHO cells.
Western Blot Analysis: A 1:1,200 dilution of this antibody detected phospho-IRS1 (Ser1101) in lysate from immunoprecipitated IRS1 transfected CHO cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.包装信息 数量 100 µL
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