- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 05-1917 描述 Anti-RSK2 Antibody, clone 4RS-1B11 Alternate Names - ribosomal protein S6 kinase
- p90RSK2
- RSK-2
背景信息 Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. 产品信息 格式 Ascites 控制 - L6 cell lysate
演示 Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide 应用 应用 This RSK2 antibody is validated for use in WB & ICC for the detection of the RSK2 protein. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry anaylsis: 1:500 dilution from a previous lot detected RSK2 in C2C12 cells. 生物信息 免疫原品种 Ovalbumin conjugated linear peptide corresponding to the N-terminus of human RSK2. 表位 N-terminus 宿主 Mouse 特异性 Antibody recognizes the N-terminus of RSK2. 同种型 IgG1κ 品种反应性 RatHumanMouse Species Reactivity Note Reacts with Human, Mouse and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - BC096303
基因符号 - MAPKAPK1B
- RPS6KA3
- CLS
- RSK2
- p90-RSK2
- ISPK1
- MRX19
- HU-3
- pp90RSK2
纯化方法 Unpurified UniProt编号 - P51812
UniProt汇总 Function Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Catalytic activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Magnesium By similarity.
Enzyme regulation Activated by multiple phosphorylations on threonine and serine residues By similarity.
Subunit structure Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation By similarity. Interacts with NFATC4. Ref.10
Tissue specificity Expressed in many tissues, highest levels in skeletal muscle.Post-translational modification Autophosphorylated on Ser-386, as part of the activation process By similarity. Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.18
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369 By similarity.
Involvement in disease Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. Ref.19 Ref.20 Ref.21
产品使用声明 质量保证 Evaluated by Western Blot in L6 cell lysate.
Western Blot Analysis: 1:1000 of this antibody detected RSK2 on 10 µg of L6 lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,OP01,Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)
下一件merck millipore产品：merck millipore,默克密理博,MABN633,Anti-a-Synuclein, clone 10D2 Antibody
相关产品
merck millipore,默克密理博,17-10398,In Vitro Vascular Permeability Imaging Assay (Green)
merck millipore,默克密理博,SF-173,GNA11; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MAB379,Anti-Glutamate Receptor 5, 6, 7 Antibody, clone 4F5
merck millipore,默克密理博,567706,Sphingomyelin, Bovine Spinal Cord - CAS 85187-10-6 - Calbiochem
merck millipore,默克密理博,OP01,Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)
merck millipore,默克密理博,MABN633,Anti-a-Synuclein, clone 10D2 Antibody
merck millipore,默克密理博,09-437,Anti-phospho-IRS1 (Ser1101) Antibody
merck millipore,默克密理博,UFC30GV00,Ultrafree-MC，GV 0.22 µm
merck millipore,默克密理博,MAB3749,Anti-LEF-1 Antibody, transactivation domain, clone 3A12
merck millipore,默克密理博,IL032,Interleukin-12 Protein, Recombinant mouse