merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB369,Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

  • merck millipore,默克密理博,MAB369,Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

    产品名称：Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt
    产品型号：MAB369
    This Anti-Dopamine Transporter Antibody, N-terminus, clone DAT-Nt is validated for use in IC, IH, WB for the detection of Dopamine Transporter.

    merck millipore,默克密理博,MAB369,Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

  • 产品介绍
  • merck millipore,默克密理博,MAB369,Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, M, MkWB, ICC, IHCRCulture SupernatantMonoclonal Antibody
    描述
    产品目录编号 MAB369
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt
    Alternate Names
    • DAT
    背景信息 The transmembrane dopamine transporter (DAT) is located on the presynaptic nerve terminal and is responsible for terminating dopaminergic transmission by transporting dopamine from the synaptic cleft into the dopaminergic neuron (reuptake). Dopaminergic pathways have been strongly implicated in reward and addiction, motivation, alcoholism, ADHD and degenerative motor diseases such as Parkinson's, Huntington's and Chorea.
    产品信息
    格式 Culture Supernatant
    控制
    • Positive Control: Brain (caudate, putamen, and nucleus accumbens) tissue
    演示 UnPurified tissue culture supernatant, filtered through a 0.2 μm membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
    应用
    应用 This Anti-Dopamine Transporter Antibody, N-terminus, clone DAT-Nt is validated for use in IC, IH, WB for the detection of Dopamine Transporter.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Western blot: Recognizes a diffuse band at approximately 70-85 kDa on western blots of extracts (20 ug total protein) from human caudate, putamen, and nucleus accumbens.

    Immunohistochemistry: 4% paraformaldehyde fixed tissue (care should be taken not to over-fix tissue); perfusion followed by less than 90 minutes post-fixation, then cryoprotect. Suggested working dilution 1:1,000-1:10,000. If using on rat tissue, absorbed anti-rat secondary antibodies are recommended, and the use of rat PAP systems, or ABC detection will enhance sensitivity.

    Immunocytochemistry: 1:5,000 to 1:10,000.

    Immunoblotting (not recommended for use on rat)

    Optimal working dilutions and protocols must be determined by end user.



    IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB369

    This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed mouse brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

    1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

    2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

    3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

    4) 3 x 10 minute washes in TBS.

    5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

    6) 3 x 10 minute washes in TBS.

    7) ABC Elite (1:200 Vector Labs) in TBS.

    8) 2 x 10 minute washes in TBS.

    9) 1 x 10 minute wash in phosphate buffer (no saline).

    10) DAB reaction with 0.06% NiCl added for intensification.

    11) 2 x 10 minute washes in PBS.

    12) 1 x 10 minute wash in phosphate buffer (no saline).
    生物信息
    免疫原品种 N-terminus of human dopamine transporter fused to Glutathione S-transferase.
    表位 N-terminus
    克隆 DAT-Nt
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rat
    特异性 Recognizes Dopamine transporter, N-terminus. Shows no cross reactivity to the closely related serotonin and norepinephrine transporters (Miller, 1997). Immunolocalization of DAT on paraformaldehyde fixed frozen sections of human brain using MAB369 shows dense punctate staining throughout the caudate, putamen and accumbens (Miller, 1997).
    同种型 IgG2aκ
    品种反应性 RatHumanMouseMonkey
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001044.3
    Entrez基因汇总 The dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM]
    基因符号
    • DAT
    • SLC6A3
    • DAT1
    • ADHD
    • ADD
    纯化方法 Unpurified
    UniProt编号
    • Q01959
    UniProt汇总 FUNCTION: SwissProt: Q01959 # Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
    SIZE: 620 amino acids; 68495 Da
    SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1.
    SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
    SIMILARITY: SwissProt: Q01959 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
    MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB369,Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

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