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merck millipore,默克密理博,MAB1569,Anti-Microglia Antibody, clone 25F9
产品名称：Anti-Microglia Antibody, clone 25F9
产品型号：MAB1569
Anti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH.
merck millipore,默克密理博,MAB1569,Anti-Microglia Antibody, clone 25F9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB1569 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Microglia Antibody, clone 25F9 Alternate Names - Late State Inflammatory
- Macrophages
背景信息 ANTIGEN DISTRIBUTION:
Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.产品信息 格式 Purified 演示 Purified immunoglobulin from culture supernatant. Lyophilized from PBS, pH 7.2 containing 5 mg/mL BSA and 0.09% sodium azide. Reconstitute with 500 μL of sterile distilled water. 应用 应用 Anti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Immunocytochemistry
应用说明 Immunohistochemistry: 1:50-1:200 using human tonsil cryosection. The antibody is reactive on formalin fixed / paraffin sections. Enzyme digestion (proteinase pretreatement) is recommended.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.生物信息 克隆 25F9 宿主 Mouse 特异性 Human late stage inflammatory macrophages. The monoclonal is suitable for use on BAL (bronchial lavage fluids) and other lavages. The antigen recognized by MAB1569 is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. The antibody has also been reported to recognize microglia stem cells (X International Congress of Neuropathology, Stockholm, le 11 September,1986.PosterA-701).
Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.
Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at 2-8°C for up to 6 months. After reconstitution maintain at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB2255,Anti-Tropomyosin-3 Antibody, clone 3F8.2
下一件merck millipore产品：merck millipore,默克密理博,SF-2670,SLC25A12 ; Human, Cyanine 5 RNA Detection Probe,SmartFlare
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